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验证数据展示

  • IF Staining of HeLa using CL488-68155

    IF Staining of HeLa using CL488-68155

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 STIP1 antibody (CL488-68155, Clone: 1B5H2 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated STIP1 Monoclonal antibody

STIP1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68155

产品说明书

CloneNo. 1B5H2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STIP1 Ready-To-Use IHC Kit. STIP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68155-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68155 targets STIP1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 STIP1 fusion protein Ag7851 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stress-induced-phosphoprotein 1
别名 HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1
计算分子量 63 kDa
观测分子量61 kDa
GenBank蛋白编号BC002987
基因名称 STIP1
Gene ID (NCBI) 10963
RRIDAB_3672976
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31948
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STIP1 antibody CL488-68155Download protocol
Standard Protocols
Click here to view our Standard Protocols