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验证数据展示

  • IF Staining of HeLa using CL488-10348

    IF Staining of HeLa using CL488-10348

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 STOML2 antibody (CL488-10348) at dilution of 1:200.

  • IF Staining of HeLa using CL488-10348

    IF Staining of HeLa using CL488-10348

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 STOML2 antibody (CL488-10348) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated STOML2 Polyclonal antibody

STOML2 Polyclonal Antibody for IF/ICC
Cat No. CL488-10348

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

SLP2, SLP 2, Paratarg-7, Paraprotein target 7, EPB72-like protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10348-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10348 targets STOML2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 STOML2 fusion protein Ag0363 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stomatin (EPB72)-like 2
别名 SLP2, SLP 2, Paratarg-7, Paraprotein target 7, EPB72-like protein 2
计算分子量 356 aa, 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC002442
基因名称 STOML2
Gene ID (NCBI) 30968
RRIDAB_3083873
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJZ1
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STOML2 antibody CL488-10348Download protocol
Standard Protocols
Click here to view our Standard Protocols