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  • KD/KO Validated

STRAP Polyclonal antibody

STRAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18277-1-AP

Print datasheet

Synonyms

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STRAP Ready-To-Use IHC Kit. STRAP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18277-1-AP targets STRAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, hamster
Immunogen STRAP fusion protein Ag13085 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine/threonine kinase receptor associated protein
Synonyms MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC000162
Gene Symbol STRAP
Gene ID (NCBI) 11171
RRIDAB_2255489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3F4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.

实验方案

Product Specific Protocols
WB protocol for STRAP antibody 18277-1-APDownload protocol
IHC protocol for STRAP antibody 18277-1-APDownload protocol
IF protocol for STRAP antibody 18277-1-APDownload protocol
IP protocol for STRAP antibody 18277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nucleic Acids Res

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Authors - Evan P Booy
  • KD Validated
humanWB,IP

EMBO J

The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation.

Authors - Muhammad Riaz Khan
  • KD Validated
human

Cancers (Basel)

Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma Cell Lines.

Authors - Laura V Bownes
  • KD Validated
humanWB

Front Oncol

STRAP as a New Therapeutic Target for Poor Prognosis of Pancreatic Ductal Adenocarcinoma Patients Mainly Caused by TP53 Mutation.

Authors - Shanshan Hu
  • KD Validated
humanWB

J Biol Chem

The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.

Authors - Evan P Booy
humanWB,IF

Int J Mol Sci

SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability.

Authors - Miao Yu