• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HeLa using CL488-67154

    FC experiment of HeLa using CL488-67154

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SUMO2/3 (CL488-67154, Clone:1A1B3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using CL488-67154

    IF Staining of HeLa using CL488-67154

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SUMO2/3 antibody (CL488-67154, Clone: 1A1B3 ) at dilution of 1:200, CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SUMO2/3 Monoclonal antibody

SUMO2/3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67154

产品说明书

CloneNo. 1A1B3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

SUMO2, 1A1B3, HSMT3, Sentrin 2, Sentrin2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67154-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67154 targets SUMO2/3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SUMO2/3 fusion protein Ag28672 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
别名 SUMO2, 1A1B3, HSMT3, Sentrin 2, Sentrin2
计算分子量 11 kDa
观测分子量 18 kDa
GenBank蛋白编号BC008450
基因名称 SUMO2
Gene ID (NCBI) 6613
RRIDAB_2919430
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61956
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SUMO2/3 antibody CL488-67154Download protocol
FC protocol for CL Plus 488 SUMO2/3 antibody CL488-67154Download protocol
Standard Protocols
Click here to view our Standard Protocols