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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated SUMO2/3 Monoclonal antibody

SUMO2/3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1A1B3

Cat no : CL555-67154

Print datasheet

Synonyms

SUMO2, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2, Sentrin 2

验证数据展示

  • FC experiment of Jurkat using CL555-67154

    FC experiment of Jurkat using CL555-67154

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SUMO2/3 (CL555-67154, Clone:1A1B3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 67154-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67154 targets SUMO2/3 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen SUMO2/3 fusion protein Ag28672 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Synonyms SUMO2, Small ubiquitin-related modifier 2, Sentrin-2, Sentrin2, Sentrin 2
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC008450
Gene Symbol SUMO2
Gene ID (NCBI) 6613
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61956
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

实验方案

Product Specific Protocols
FC protocol for CL555 SUMO2/3 antibody CL555-67154Download protocol
Standard Protocols
Click here to view our Standard Protocols