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SYAP1 Monoclonal antibody

SYAP1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E12A5

Cat no : 68410-1-Ig

Print datasheet

Synonyms

DKFZp686K221, FLJ14495, FLJ44185, PRO3113, SYAP1, Synapse associated protein 1

验证数据展示

  • WB analysis using 68410-1-Ig

    WB analysis using 68410-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68410-1-Ig (SYAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of L02 using 68410-1-Ig

    IP experiment of L02 using 68410-1-Ig

    IP result of anti-SYAP1 (IP:68410-1-Ig, 4ug; Detection:68410-1-Ig 1:2000) with L02 cells lysate 3080 ug.

  • IF Staining of HepG2 using 68410-1-Ig

    IF Staining of HepG2 using 68410-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYAP1 antibody (68410-1-Ig, Clone: 2E12A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inL02 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68410-1-Ig targets SYAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SYAP1 fusion protein Ag9626 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name synapse associated protein 1, SAP47 homolog (Drosophila)
Synonyms DKFZp686K221, FLJ14495, FLJ44185, PRO3113, SYAP1, Synapse associated protein 1
Calculated Molecular Weight 352 aa, 40 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC014657
Gene Symbol SYAP1
Gene ID (NCBI) 94056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96A49
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

实验方案

Product Specific Protocols
WB protocol for SYAP1 antibody 68410-1-IgDownload protocol
IF protocol for SYAP1 antibody 68410-1-IgDownload protocol
IP protocol for SYAP1 antibody 68410-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols