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验证数据展示

  • IF Staining of HepG2 using CL488-68410

    IF Staining of HepG2 using CL488-68410

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SYAP1 antibody (CL488-68410, Clone: 2E12A5 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated SYAP1 Monoclonal antibody

SYAP1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68410

Print datasheet

CloneNo. 2E12A5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68410-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68410 targets SYAP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen SYAP1 fusion protein Ag9626 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name synapse associated protein 1, SAP47 homolog (Drosophila)
Synonyms
Calculated Molecular Weight 352 aa, 40 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC014657
Gene Symbol SYAP1
Gene ID (NCBI) 94056
RRIDAB_3673008
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96A49
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SYAP1 antibody CL488-68410Download protocol
Standard Protocols
Click here to view our Standard Protocols