Sam50 Polyclonal antibody
Sam50 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (2)
Applications
IF/ICC, IHC, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, mouse heart tissur, rat heart tissue |
Positive IHC detected in | human heart tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28679-1-AP targets Sam50 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | IF/ICC, IHC, WB,ELISA Application Description |
Cited Applications | WB, IP, IF |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | mouse, rat |
Immunogen | Sam50 fusion protein Ag29152 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | sorting and assembly machinery component 50 homolog (S. cerevisiae) |
Synonyms | CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, SAM50, SAMM50, TOB55, TRG 3, TRG3, YNL026W |
Calculated Molecular Weight | 469 aa, 52 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC007830 |
Gene Symbol | SAMM50 |
Gene ID (NCBI) | 25813 |
RRID | AB_2881192 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y512 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Sam50 antibody 28679-1-AP | Download protocol |
IHC protocol for Sam50 antibody 28679-1-AP | Download protocol |
IF protocol for Sam50 antibody 28679-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Cardiovasc Med Samm50 Promotes Hypertrophy by Regulating Pink1-Dependent Mitophagy Signaling in Neonatal Cardiomyocytes | ||
Int J Biol Sci Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients | ||
Cell Signal Mitochondrial outer membrane protein Samm50 protects against hypoxia-induced cardiac injury by interacting with Shmt2 |