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验证数据展示

  • IF Staining of HeLa using CL488-28679

    IF Staining of HeLa using CL488-28679

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Sam50 antibody (CL488-28679) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-28679

    FC experiment of HeLa using CL488-28679

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated Sam50 Polyclonal antibody (CL488-28679)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28679

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

SAMM50, TOB55, SAMM 50, OMP85, CGI-51

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 28679-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28679 targets Sam50 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Sam50 fusion protein Ag29152 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Synonyms SAMM50, TOB55, SAMM 50, OMP85, CGI-51
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007830
Gene Symbol Sam50
Gene ID (NCBI) 25813
RRIDAB_3672832
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y512
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Sam50 antibody CL488-28679Download protocol
FC protocol for CL Plus 488 Sam50 antibody CL488-28679Download protocol
Standard Protocols
Click here to view our Standard Protocols