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CoraLite® Plus 488-conjugated Sodium iodide symporter Polyclonal antibody

Sodium iodide symporter Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24324

Print datasheet

Synonyms

NIS, Sodium-iodide symporter (Na(+)/I(-) symporter), SLC5A5, Natrium iodide transporter

验证数据展示

  • FC experiment of MCF-7 using CL488-24324

    FC experiment of MCF-7 using CL488-24324

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Sodium iodide symporter (CL488-24324) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 24324-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24324 targets Sodium iodide symporter in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Sodium iodide symporter fusion protein Ag19504 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 5 (sodium iodide symporter), member 5
Synonyms NIS, Sodium-iodide symporter (Na(+)/I(-) symporter), SLC5A5, Natrium iodide transporter
Calculated Molecular Weight 643 aa, 69 kDa
Observed Molecular Weight 50-55 kDa, 75-100 kDa
GenBank Accession NumberBC105047
Gene Symbol Sodium iodide symporter
Gene ID (NCBI) 6528
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92911
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The sodium iodide symporter (Na+/I - symporter, NIS), encoded by SLC5A5, is an integral plasma membrane glycoprotein that plays an important role in iodide uptake by thyroid cells. Expression of sodium iodide symporter has also been found in extra-thyroidal tissues, including gastric mucosa, lactating mammary gland and salivary glands. Increased expression of sodium iodide symporter has been found in thyroid tissue from patients with Graves' disease as well as papillary thyroid carcinomas. In addition, sodium iodide symporter was found to express in majority of breast cancer tissue but not in normal tissue. Sodium iodide symporter can be a promising diagnostic and therapeutic tool for thyroid cancer and breast cancer. This antibody recognizes the mature approximately 75-100 kDa protein and a partially glycosylated 50-55 kDa protein. (PMID: 12588808, 9525971)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Sodium iodide symporter antibody CL488-24324Download protocol
Standard Protocols
Click here to view our Standard Protocols