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验证数据展示

  • FC experiment of A549 using CL488-11850

    FC experiment of A549 using CL488-11850

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Surfactant Protein A (CL488-11850)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Surfactant Protein A Polyclonal antibody

Surfactant Protein A Polyclonal Antibody for FC (Intra)
Cat No. CL488-11850

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

Alveolar proteinosis protein, COLEC4, Collectin 4, FLJ51913, PSAP, PSP A, PSPA, SFTP1, SFTPA, SFTPA1, SFTPA1B, SFTPA2, SP A, SP A1, Surfactant Protein A, surfactant protein A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA549 cells
Positive FC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy SFTPA1 Ready-To-Use IHC Kit. SFTPA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11850-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11850 targets Surfactant Protein A in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Surfactant Protein A fusion protein Ag2417 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 surfactant protein A1
别名 Alveolar proteinosis protein, COLEC4, Collectin 4, FLJ51913, PSAP, PSP A, PSPA, SFTP1, SFTPA, SFTPA1, SFTPA1B, SFTPA2, SP A, SP A1, Surfactant Protein A, surfactant protein A1
计算分子量 248 aa, 26 kDa
GenBank蛋白编号BC026229
基因名称 Surfactant Protein A
Gene ID (NCBI) 653509
RRIDAB_3672515
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWL2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Surfactant protein A (SP-A), is the major protein component of pulmonary surfactant involved in surfactant-related function or structure and in the regulation of inflammatory processes and innate host defens. In humans and primates, SP-A1 (SFTPA1) and SP-A2 (SFTPA2) genes encode SP-A, and these two SP-A genes have high homology (PMID: 34484180, PMID: 19392648). This antibody can recognize both SP-A1 (SFTPA1) and SP-A2 (SFTPA2).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Surfactant Protein A antibody CL488-11850Download protocol
Standard Protocols
Click here to view our Standard Protocols