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Host
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Species Reactivity
0
Species Reactivity
0
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Conjugate
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验证数据展示

  • WB analysis using 82900-1-RR (same clone as 82900-1-PBS)

    WB analysis using 82900-1-RR (same clone as 82900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82900-1-RR (Synaptophysin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 82900-1-RR (same clone as 82900-1-PBS)

    IP experiment of mouse brain using 82900-1-RR (same clone as 82900-1-PBS)

    IP result of anti-Synaptophysin (IP:82900-1-RR, 4ug; Detection:82900-1-RR 1:5000) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

    IHC staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IHC staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

    IHC staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IF Staining of PC-12 using 82900-1-RR (same clone as 82900-1-PBS)

    IF Staining of PC-12 using 82900-1-RR (same clone as 82900-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Synaptophysin antibody (82900-1-RR, Clone: 2H14 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 82900-1-RR (same clone as 82900-1-PBS)

    FC experiment of A549 using 82900-1-RR (same clone as 82900-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Synaptophysin Recombinant antibody (82900-1-RR, Clone:2H14) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82900-1-RR (same clone as 82900-1-PBS)

    IHC staining of mouse brain using 82900-1-RR (same clone as 82900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Synaptophysin antibody included
  • IF Staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

    IF Staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Synaptophysin antibody (82900-1-RR, Clone: 2H14 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

    IF Staining of mouse pancreas using 82900-1-RR (same clone as 82900-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Synaptophysin antibody (82900-1-RR, Clone: 2H14 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82900-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82900-1-RR

    Affinity and Kinetic Characterization of 82900-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82900-1-RR against Human Synaptophysin were performed. The affinity constant is below 1 pM.

Synaptophysin Recombinant antibody, PBS Only

Synaptophysin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 82900-1-PBS

产品说明书

CloneNo. 2H14

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

别名

SYP, SYN, MRX96, 2H14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.

产品信息

82900-1-PBS targets Synaptophysin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Synaptophysin fusion protein Ag11781 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptophysin
别名 SYP, SYN, MRX96, 2H14
计算分子量 313 aa, 34 kDa
观测分子量38-40 kDa
GenBank蛋白编号BC064550
基因名称 Synaptophysin
Gene ID (NCBI) 6855
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08247
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)