Synaptophysin Recombinant antibody
Synaptophysin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
2H14
验证数据展示
经过测试的应用
Positive WB detected in | PC-12 cells, human cerebellum tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-12 cells, U2OS cells |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82900-1-RR targets Synaptophysin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Synaptophysin fusion protein Ag11781 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | synaptophysin |
Synonyms | SYP, SYN, MRX96, 2H14 |
Calculated Molecular Weight | 313 aa, 34 kDa |
Observed Molecular Weight | 38-40 kDa |
GenBank Accession Number | BC064550 |
Gene Symbol | Synaptophysin |
Gene ID (NCBI) | 6855 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08247 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Synaptophysin antibody 82900-1-RR | Download protocol |
IHC protocol for Synaptophysin antibody 82900-1-RR | Download protocol |
IF protocol for Synaptophysin antibody 82900-1-RR | Download protocol |
IP protocol for Synaptophysin antibody 82900-1-RR | Download protocol |
FC protocol for Synaptophysin antibody 82900-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |