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验证数据展示

  • IF Staining of mouse brain using CL488-82900

    IF Staining of mouse brain using CL488-82900

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-82900, Clone: 2H14 ) at dilution of 1:200, Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-12 using CL488-82900

    IF Staining of PC-12 using CL488-82900

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 Synaptophysin antibody (CL488-82900, Clone: 2H14 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated Synaptophysin Recombinant antibody

Synaptophysin Recombinant Antibody for IF/ICC, IF-P
Cat No. CL488-82900

产品说明书

CloneNo. 2H14

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P

别名

SYP, SYN, MRX96, 2H14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82900-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82900 targets Synaptophysin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Synaptophysin fusion protein Ag11781 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptophysin
别名 SYP, SYN, MRX96, 2H14
计算分子量 313 aa, 34 kDa
观测分子量38-40 kDa
GenBank蛋白编号BC064550
基因名称 Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_3673105
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08247
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Synaptophysin antibody CL488-82900Download protocol
Standard Protocols
Click here to view our Standard Protocols