验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells. HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue |
Positive IF/ICC detected in | T-47D cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
68164-1-Ig targets TCEB1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | Human, mouse, rat |
文献引用反应性 | human |
免疫原 | TCEB1 fusion protein Ag22601 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C) |
别名 | EloC, Elongin 15 kDa subunit, Elongin C, SIII, SIII p15, TCEB1 |
计算分子量 | 112 aa, 12 kDa |
观测分子量 | 12 kDa |
GenBank蛋白编号 | BC013809 |
基因名称 | TCEB1 |
Gene ID (NCBI) | 6921 |
RRID | AB_2923683 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q15369 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).
实验方案
Product Specific Protocols | |
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WB protocol for TCEB1 antibody 68164-1-Ig | Download protocol |
IF protocol for TCEB1 antibody 68164-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |