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TCEB1 Monoclonal antibody

TCEB1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D6D8

Cat no : 68164-1-Ig

Print datasheet

Synonyms

EloC, Elongin 15 kDa subunit, Elongin C, SIII, SIII p15, TCEB1

验证数据展示

  • WB analysis using 68164-1-Ig

    WB analysis using 68164-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68164-1-Ig (TCEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of T-47D using 68164-1-Ig

    IF Staining of T-47D using 68164-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed T-47D cells using TCEB1 antibody (68164-1-Ig, Clone: 2D6D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells. HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inT-47D cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68164-1-Ig targets TCEB1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen TCEB1 fusion protein Ag22601 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
Synonyms EloC, Elongin 15 kDa subunit, Elongin C, SIII, SIII p15, TCEB1
Calculated Molecular Weight 112 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC013809
Gene Symbol TCEB1
Gene ID (NCBI) 6921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15369
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).

实验方案

Product Specific Protocols
WB protocol for TCEB1 antibody 68164-1-IgDownload protocol
IF protocol for TCEB1 antibody 68164-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A

Authors - Mingchao Mu
View Article