验证数据展示
经过测试的应用
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-65106 targets TCR Beta in IF/ICC, FC applications and shows reactivity with Mouse samples.
| Tested Applications | IF/ICC, FC Application Description |
| Cited Applications | IF |
| Tested Reactivity | Mouse |
| Immunogen | Affinity purified TCR from mouse DO-11.10 cells 种属同源性预测 |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | T-cell receptor beta chain |
| Synonyms | T cell receptor beta chain, TCR Beta, Tcrb, TCRβ, Tib |
| Gene Symbol | TCR beta |
| Gene ID (NCBI) | 21577 |
| RRID | AB_2883180 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
T-cell receptor (TCR) consists of heterodimeric glycoproteins (TCR alpha/beta or gamma/delta) that demonstrate homology with immunoglobulins (PMID: 2144901). TCR beta is a member of the immunoglobulin superfamily and is expressed on alpha/beta TCR-bearing T cells and thymocytes. The TCR heterodimers are noncovalently associated with CD3. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta (PMID: 1826255). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TCR Beta antibody CL488-65106 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |