验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-65106 targets TCR Beta in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Mouse |
| Immunogen | Affinity purified TCR from mouse DO-11.10 cells 种属同源性预测 |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | T-cell receptor beta chain |
| Synonyms | T cell receptor beta chain, TCR Beta, Tcrb, TCRβ, Tib |
| Gene Symbol | TCR beta |
| Gene ID (NCBI) | 21577 |
| RRID | AB_2883684 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
T-cell receptor (TCR) consists of heterodimeric glycoproteins (TCR alpha/beta or gamma/delta) that demonstrate homology with immunoglobulins (PMID: 2144901). TCR beta is a member of the immunoglobulin superfamily and is expressed on alpha/beta TCR-bearing T cells and thymocytes. The TCR heterodimers are noncovalently associated with CD3. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta (PMID: 1826255). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 TCR Beta antibody CL647-65106 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |