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验证数据展示

  • IHC staining of human rectal cancer using 84636-1-RR (same clone as 84636-1-PBS)

    IHC staining of human rectal cancer using 84636-1-RR (same clone as 84636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84636-1-RR (TERT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84636-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84636-1-RR (same clone as 84636-1-PBS)

    IF Staining of MCF-7 using 84636-1-RR (same clone as 84636-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TERT antibody (84636-1-RR, Clone: 241074H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84636-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84636-1-RR (same clone as 84636-1-PBS)

    FC experiment of HeLa using 84636-1-RR (same clone as 84636-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TERT Recombinant antibody (84636-1-RR, Clone:241074H2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84636-1-PBS in a different storage buffer formulation.

TERT Recombinant antibody, PBS Only

TERT Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84636-1-PBS

产品说明书

CloneNo. 241074H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

TCS1, hTERT, hEST2, EST2, EC:2.7.7.49

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84636-1-PBS targets TERT in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 telomerase reverse transcriptase
别名 TCS1, hTERT, hEST2, EST2, EC:2.7.7.49
计算分子量 127 kDa
GenBank蛋白编号NM_198255
基因名称 TERT
Gene ID (NCBI) 7015
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO14746
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Telomerase reverse transcriptase (TERT) is a catalytic subunit of telomerase. TERT plays a key role in cancer formation, ensuring chromosomal stability by maintaining telomere length, and allowing cells to avert senescence. It constitutes a limiting factor for formation of the telomerase complex in cancer cells. TERT is one of two major components of the larger telomerase complex, which extends telomeres by adding specific short repetitive DNA sequences. Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells.