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TERT Recombinant antibody

TERT Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241074H2

Cat no : 84636-1-RR

Print datasheet

Synonyms

TCS1, hTERT, hEST2, EST2, EC:2.7.7.49

验证数据展示

  • IF Staining of MCF-7 using 84636-1-RR

    IF Staining of MCF-7 using 84636-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TERT antibody (84636-1-RR, Clone: 241074H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84636-1-RR

    FC experiment of HeLa using 84636-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TERT Recombinant antibody (84636-1-RR, Clone:241074H2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84636-1-RR targets TERT in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name telomerase reverse transcriptase
Synonyms TCS1, hTERT, hEST2, EST2, EC:2.7.7.49
Calculated Molecular Weight 127 kDa
GenBank Accession NumberNM_198255
Gene Symbol TERT
Gene ID (NCBI) 7015
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14746
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Telomerase reverse transcriptase (TERT) is a catalytic subunit of telomerase. TERT plays a key role in cancer formation, ensuring chromosomal stability by maintaining telomere length, and allowing cells to avert senescence. It constitutes a limiting factor for formation of the telomerase complex in cancer cells. TERT is one of two major components of the larger telomerase complex, which extends telomeres by adding specific short repetitive DNA sequences. Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells.

实验方案

Product Specific Protocols
IF protocol for TERT antibody 84636-1-RRDownload protocol
FC protocol for TERT antibody 84636-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols