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CoraLite® Plus 488-conjugated TFAP4 Recombinant antibody

TFAP4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240031D5

Cat no : CL488-83196-4

Print datasheet

Synonyms

Transcription factor AP 4, Class C basic helix-loop-helix protein 41, bHLHc41, AP 4, Activating enhancer-binding protein 4

验证数据展示

  • IF Staining of Hela using CL488-83196-4

    IF Staining of Hela using CL488-83196-4

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 TFAP4 antibody (CL488-83196-4, Clone: 240031D5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83196-4

    FC experiment of HeLa using CL488-83196-4

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated TFAP4 Recombinant antibody (CL488-83196-4, Clone:240031D5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 83196-4-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-83196-4 is TFAP4 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen TFAP4 fusion protein Ag2633 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transcription factor AP-4 (activating enhancer binding protein 4)
Synonyms Transcription factor AP 4, Class C basic helix-loop-helix protein 41, bHLHc41, AP 4, Activating enhancer-binding protein 4
Calculated Molecular Weight 338 aa, 39 kDa
Observed Molecular Weight39-42 kDa
GenBank Accession NumberBC010576
Gene Symbol TFAP4
Gene ID (NCBI) 7023
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01664
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TFAP4 antibody CL488-83196-4Download protocol
FC protocol for CL Plus 488 TFAP4 antibody CL488-83196-4Download protocol
Standard Protocols
Click here to view our Standard Protocols