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验证数据展示

  • IF Staining of Hela using CL488-83196-4

    IF Staining of Hela using CL488-83196-4

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 TFAP4 antibody (CL488-83196-4, Clone: 240031D5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83196-4

    FC experiment of HeLa using CL488-83196-4

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated TFAP4 Recombinant antibody (CL488-83196-4, Clone:240031D5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated TFAP4 Recombinant antibody

TFAP4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83196-4

产品说明书

CloneNo. 240031D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Transcription factor AP 4, Class C basic helix-loop-helix protein 41, bHLHc41, AP 4, Activating enhancer-binding protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 83196-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83196-4 targets TFAP4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 TFAP4 fusion protein Ag2633 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor AP-4 (activating enhancer binding protein 4)
别名 Transcription factor AP 4, Class C basic helix-loop-helix protein 41, bHLHc41, AP 4, Activating enhancer-binding protein 4
计算分子量 338 aa, 39 kDa
观测分子量39-42 kDa
GenBank蛋白编号BC010576
基因名称 TFAP4
Gene ID (NCBI) 7023
RRIDAB_3673220
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01664
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TFAP4 antibody CL488-83196-4Download protocol
FC protocol for CL Plus 488 TFAP4 antibody CL488-83196-4Download protocol
Standard Protocols
Click here to view our Standard Protocols