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TGIF2 Monoclonal antibody

TGIF2 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D6B6

Cat no : 67576-1-Ig

Print datasheet

Synonyms

TGFB induced factor homeobox 2, TGFB induced factor 2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2, 5 TG 3 interacting factor 2

验证数据展示

  • WB analysis using 67576-1-Ig

    WB analysis using 67576-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 67576-1-Ig

    WB analysis of COLO 320 using 67576-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 67576-1-Ig

    WB analysis of THP-1 using 67576-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 67576-1-Ig

    FC experiment of HepG2 using 67576-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TGIF2 Monoclonal antibody (67576-1-Ig, Clone:2D6B6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHepG2 cells, THP-1 cells, COLO 320 cells, Ramos cells, Daudi cells, K-562 cells, Jurkat cells, HL-60 cells, MOLT-4 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67576-1-Ig targets TGIF2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TGIF2 fusion protein Ag30083 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TGFB-induced factor homeobox 2
Synonyms TGFB induced factor homeobox 2, TGFB induced factor 2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2, 5 TG 3 interacting factor 2
Calculated Molecular Weight 237 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC012816
Gene Symbol TGIF2
Gene ID (NCBI) 60436
RRIDAB_2882789
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZN2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGIF2, is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein.

实验方案

Product Specific Protocols
WB protocol for TGIF2 antibody 67576-1-IgDownload protocol
FC protocol for TGIF2 antibody 67576-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bosn J Basic Med Sci

Circ-RNF13, as an oncogene, regulates malignant progression of HBV-associated hepatocellular carcinoma cells and HBV infection through ceRNA pathway of circ-RNF13/miR-424-5p/TGIF2.

Authors - Yan Chen
View Article