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验证数据展示

  • WB analysis using 67576-1-Ig (same clone as 67576-1-PBS)

    WB analysis using 67576-1-Ig (same clone as 67576-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67576-1-PBS in a different storage buffer formulation.

  • WB analysis of COLO 320 using 67576-1-Ig (same clone as 67576-1-PBS)

    WB analysis of COLO 320 using 67576-1-Ig (same clone as 67576-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67576-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 67576-1-Ig (same clone as 67576-1-PBS)

    WB analysis of THP-1 using 67576-1-Ig (same clone as 67576-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67576-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67576-1-Ig (same clone as 67576-1-PBS)

    FC experiment of HepG2 using 67576-1-Ig (same clone as 67576-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TGIF2 Monoclonal antibody (67576-1-Ig, Clone:2D6B6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67576-1-PBS in a different storage buffer formulation.

TGIF2 Monoclonal antibody, PBS Only

TGIF2 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 67576-1-PBS

产品说明书

CloneNo. 2D6B6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Indirect ELISA, WB

别名

TGFB induced factor homeobox 2, TGFB induced factor 2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2, 5 TG 3 interacting factor 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67576-1-PBS targets TGIF2 in Indirect ELISA, WB applications and shows reactivity with human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 human
免疫原 TGIF2 fusion protein Ag30083 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TGFB-induced factor homeobox 2
别名 TGFB induced factor homeobox 2, TGFB induced factor 2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2, 5 TG 3 interacting factor 2
计算分子量 237 aa, 26 kDa
观测分子量 30 kDa
GenBank蛋白编号BC012816
基因名称 TGIF2
Gene ID (NCBI) 60436
RRIDAB_2882789
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9GZN2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TGIF2, is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein.