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验证数据展示

  • WB analysis using 67831-1-Ig

    WB analysis using 67831-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human lung cancer using 67831-1-Ig

    IHC staining of human lung cancer using 67831-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD141/Thrombomodulin antibody included
  • IHC staining of human lung cancer using 67831-1-Ig

    IHC staining of human lung cancer using 67831-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD141/Thrombomodulin antibody included
  • IHC staining of human tonsillitis using 67831-1-Ig

    IHC staining of human tonsillitis using 67831-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67831-1-Ig (CD141/Thrombomodulin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD141/Thrombomodulin antibody included

CD141/Thrombomodulin Monoclonal antibody

CD141/Thrombomodulin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67831-1-Ig

产品说明书

CloneNo. 5A4C5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, human placenta tissue, THP-1 cells, HUVEC cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD141/Thrombomodulin Ready-To-Use IHC Kit. CD141/Thrombomodulin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67831-1-Ig targets CD141/Thrombomodulin in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CD141/Thrombomodulin fusion protein Ag5723 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 thrombomodulin
别名 THBD, Thrombomodulin, THRM, Fetomodulin, CD141
计算分子量 60 kDa
观测分子量105 kDa
GenBank蛋白编号BC035602
基因名称 THBD
Gene ID (NCBI) 7056
RRIDAB_2918593
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07204
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thrombomodulin, also known as THBD and CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

实验方案

Product Specific Protocols
WB protocol for CD141/Thrombomodulin antibody 67831-1-IgDownload protocol
IHC protocol for CD141/Thrombomodulin antibody 67831-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols