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CoraLite® Plus 488-conjugated CD141/Thrombomodulin Monoclonal antibody

CD141/Thrombomodulin Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5A4C5

Cat no : CL488-67831

Print datasheet

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

验证数据展示

  • IF Staining of human lung cancer using CL488-67831

    IF Staining of human lung cancer using CL488-67831

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 THBD antibody (CL488-67831, Clone: 5A4C5 ) at dilution of 1:200.

  • IF Staining of human lung cancer using CL488-67831

    IF Staining of human lung cancer using CL488-67831

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 THBD antibody (CL488-67831, Clone: 5A4C5 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman lung cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 67831-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67831 targets CD141/Thrombomodulin in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen CD141/Thrombomodulin fusion protein Ag5723 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name thrombomodulin
Synonyms THBD, Thrombomodulin, THRM, Fetomodulin, CD141
Calculated Molecular Weight 60 kDa
Observed Molecular Weight105 kDa
GenBank Accession NumberBC035602
Gene Symbol THBD
Gene ID (NCBI) 7056
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07204
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD141/Thrombomodulin antibody CL488-67831Download protocol
Standard Protocols
Click here to view our Standard Protocols