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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TLE1 Monoclonal antibody

TLE1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F2H11

Cat no : CL488-66216

Print datasheet

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

验证数据展示

  • IF Staining of MCF-7 using CL488-66216

    IF Staining of MCF-7 using CL488-66216

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 TLE1 antibody (CL488-66216, Clone: 2F2H11 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66216-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66216 targets TLE1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen TLE1 fusion protein Ag21327 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Synonyms E(Sp1) homolog, ESG, ESG1, GRG1, TLE1
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC010100
Gene Symbol TLE1
Gene ID (NCBI) 7088
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04724
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TLE1 antibody CL488-66216Download protocol
Standard Protocols
Click here to view our Standard Protocols