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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated TLE1 Monoclonal antibody

TLE1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2F2H11

Cat No : CL750-66216

Print datasheet

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

验证数据展示

  • WB analysis using CL750-66216

    WB analysis using CL750-66216

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66216 (TLE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 66216-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66216 targets TLE1 in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen TLE1 fusion protein Ag21327 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Synonyms E(Sp1) homolog, ESG, ESG1, GRG1, TLE1
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC010100
Gene Symbol TLE1
Gene ID (NCBI) 7088
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04724
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 TLE1 antibody CL750-66216Download protocol
Standard Protocols
Click here to view our Standard Protocols