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Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody

TLR4/CD284 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

230463C7

Cat no : 98018-1-RR

Print datasheet

Synonyms

Tlr4, CD284, toll like receptor 4, 230463C7

验证数据展示

  • FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

    FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.

  • FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

    FC experiment of Balb/c mouse peritoneal macrophages using 98018-1-RR

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (98018-1-RR, Clone: 230463C7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inBalb/c mouse peritoneal macrophages

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98018-1-RR is TLR4/CD284 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen TLR4/CD284 fusion protein Ag32998 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name toll-like receptor 4
Synonyms Tlr4, CD284, toll like receptor 4, 230463C7
Calculated Molecular Weight96 kDa
GenBank Accession NumberNM_021297
Gene Symbol Tlr4
Gene ID (NCBI) 21898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9QUK6
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

实验方案

Product Specific Protocols
FC protocol for TLR4/CD284 antibody 98018-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols