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CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody

TLR4/CD284 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230463C7

Cat no : CL647-98018

Print datasheet

Synonyms

Tlr4, CD284, toll like receptor 4, 230463C7

验证数据展示

  • FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with FITC Plus Anti-Mouse CD11b and 0.25 ug CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (CL647-98018, Clone: 230463C7) or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    FC experiment of Balb/c mouse peritoneal macrophages using CL647-98018

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse TLR4/CD284 Rabbit Recombinant Antibody (CL647-98018, Clone: 230463C7) (red) or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inBalb/c mouse peritoneal macrophages
For other applications, we recommend the unconjugated version of this antibody, 98018-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-98018 is TLR4/CD284 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen TLR4/CD284 fusion protein Ag32998 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name toll-like receptor 4
Synonyms Tlr4, CD284, toll like receptor 4, 230463C7
Calculated Molecular Weight96 kDa
GenBank Accession NumberNM_021297
Gene Symbol Tlr4
Gene ID (NCBI) 21898
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9QUK6
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 TLR4/CD284 antibody CL647-98018Download protocol
Standard Protocols
Click here to view our Standard Protocols