验证数据展示
经过测试的应用
| Positive FC detected in | Balb/c mouse peritoneal macrophages |
For other applications, we recommend the unconjugated version of this antibody, 98018-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98018 targets TLR4/CD284 in FC applications and shows reactivity with mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | mouse |
| Immunogen | TLR4/CD284 fusion protein Ag32998 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | toll-like receptor 4 |
| Synonyms | Tlr4, CD284, toll like receptor 4, 230463C7 |
| Calculated Molecular Weight | 96 kDa |
| GenBank Accession Number | NM_021297 |
| Gene Symbol | Tlr4 |
| Gene ID (NCBI) | 21898 |
| RRID | AB_3673659 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9QUK6 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 TLR4/CD284 antibody CL647-98018 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |