• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83440-2-RR (same clone as 83440-2-PBS)

    WB analysis using 83440-2-RR (same clone as 83440-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83440-2-RR (TMEM126A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

    IF Staining of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (83440-2-RR, Clone: 240425A11 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

    FC experiment of HeLa using 83440-2-RR (same clone as 83440-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TMEM126A Recombinant antibody (83440-2-RR, Clone:240425A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83440-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00449-1

    Cytometric bead array standard curve of MP00449-1

    Cytometric bead array standard curve of MP00449-1, TMEM126A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83440-2-PBS. Detection antibody: 83440-1-PBS. Standard: Ag14705. Range: 6.25-100 ng/mL.

  • Affinity and Kinetic Characterization of 83440-2-RR

    Affinity and Kinetic Characterization of 83440-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83440-2-RR against Human TMEM126A were performed. The affinity constant is 0.589 nM.

TMEM126A Recombinant antibody, PBS Only (Capture)

TMEM126A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83440-2-PBS

产品说明书

CloneNo. 240425A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

TMEM126, TMEM, OPA7, 240425A11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83440-2-PBS targets TMEM126A as part of a matched antibody pair:

MP00449-1: 83440-2-PBS capture and 83440-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 TMEM126A fusion protein Ag14705 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 126A
别名 TMEM126, TMEM, OPA7, 240425A11
计算分子量 195 aa, 22 kDa
观测分子量20-25 kDa
GenBank蛋白编号BC007875
基因名称 TMEM126A
Gene ID (NCBI) 84233
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H061
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.