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验证数据展示

  • WB analysis using 83440-2-RR

    WB analysis using 83440-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83440-2-RR (TMEM126A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83440-2-RR

    IF Staining of HeLa using 83440-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (83440-2-RR, Clone: 240425A11 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83440-2-RR

    FC experiment of HeLa using 83440-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TMEM126A Recombinant antibody (83440-2-RR, Clone:240425A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83440-2-RR

    Affinity and Kinetic Characterization of 83440-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83440-2-RR against Human TMEM126A were performed. The affinity constant is 0.589 nM.

TMEM126A Recombinant antibody

TMEM126A Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83440-2-RR

产品说明书

CloneNo. 240425A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TMEM126, TMEM, OPA7, 240425A11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells, HeLa cells, MCF-7 cells, U-251 cells, U2OS cells, human placenta tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83440-2-RR targets TMEM126A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TMEM126A fusion protein Ag14705 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 126A
别名 TMEM126, TMEM, OPA7, 240425A11
计算分子量 195 aa, 22 kDa
观测分子量20-25 kDa
GenBank蛋白编号BC007875
基因名称 TMEM126A
Gene ID (NCBI) 84233
RRIDAB_3671081
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H061
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.

实验方案

Product Specific Protocols
WB protocol for TMEM126A antibody 83440-2-RRDownload protocol
IF protocol for TMEM126A antibody 83440-2-RRDownload protocol
FC protocol for TMEM126A antibody 83440-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols