• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TMEM132E Recombinant antibody, PBS Only (Detector)

TMEM132E Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230525E12

Cat no : 82983-2-PBS

Print datasheet

Synonyms

transmembrane protein 132E, 230525E12

验证数据展示

  • WB analysis using 82983-2-RR (same clone as 82983-2-PBS)

    WB analysis using 82983-2-RR (same clone as 82983-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82983-2-RR (TMEM132E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82983-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00054-2

    Cytometric bead array standard curve of MP00054-2

    Cytometric bead array standard curve of MP00054-2, TMEM132E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82983-1-PBS. Detection antibody: 82983-2-PBS. Standard: Ag26772. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 82983-2-RR

    Affinity and Kinetic Characterization of 82983-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82983-2-RR against Human TMEM132E were performed. The affinity constant is 0.254 nM.

产品信息

82983-2-PBS targets TMEM132E as part of a matched antibody pair:

MP00054-2: 82983-1-PBS capture and 82983-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen TMEM132E fusion protein Ag26772 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 132E
Synonyms transmembrane protein 132E, 230525E12
Calculated Molecular Weight 116 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberNM_001304438
Gene Symbol TMEM132E
Gene ID (NCBI) 124842
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6IEE7
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transmembrane protein 132E (TMEM132E) is a member of the TMEM132 family which also include TMEM132A, TMEM132B, TMEM132C, TMEM132D (PMID: 29088312). The gene of TMEM132E is located within the DFNB99 locus on chromosome 17q12 and is highly expressed in the inner ear and other tissues including brain, kidney, lung, liver, spleen, heart, small intestine, colon, thymus, and stomach (PMID: 31656313). A variant of TMEM132E has been associated with autosomal-recessive nonsyndromic hearing loss DFNB99 (PMID: 25331638).