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  • Featured Product
  • KD/KO Validated

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF/ICC,IF-P, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 19851-1-AP

Print datasheet

Synonyms

hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, STING1, TMEM173, TMEM173/STING, transmembrane protein 173

验证数据展示

  • WB analysis using 19851-1-AP

    WB analysis using 19851-1-AP

    Various lysis were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 19851-1-AP

    WB analysis of THP-1 using 19851-1-AP

    WB result of TMEM173/STING antibody (19851-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells.

  • WB analysis of HepG2 using 19851-1-AP

    WB analysis of HepG2 using 19851-1-AP

    WB result of TMEM173/STING antibody (19851-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and TMEM173/STING knockout HepG2 cells.

  • IHC staining of human tonsillitis using 19851-1-AP

    IHC staining of human tonsillitis using 19851-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STING antibody included
  • IHC staining of human tonsillitis using 19851-1-AP

    IHC staining of human tonsillitis using 19851-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STING antibody included
  • IP experiment of mouse spleen using 19851-1-AP

    IP experiment of mouse spleen using 19851-1-AP

    IP result of anti-TMEM173/STING (IP:19851-1-AP, 4ug; Detection:19851-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

  • IF Staining of HT-29 using 19851-1-AP

    IF Staining of HT-29 using 19851-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of THP-1 using 19851-1-AP

    FC experiment of THP-1 using 19851-1-AP

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TMEM173/STING (19851-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, HepG2 cells, Raji cells, mouse spleen tissue, THP-1 cells, HL-60 cells, mouse testis tissue, HT-29 cells, mouse thymus tissue, C2C12 cells
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, rat thymus tissue, mouse thymus tissue, human spleen tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHT-29 cells, human tonsillitis tissue, THP-1 cells
Positive FC detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19851-1-AP targets TMEM173/STING in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC,IF-P, IHC, IP, WB, ELISA
Cited ApplicationsWB,IP,IHC,IF,FC,CoIP,Blocking assay
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Immunogen TMEM173/STING fusion protein Ag13921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 173
Synonyms hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, STING1, TMEM173, TMEM173/STING, transmembrane protein 173
Calculated Molecular Weight 379 aa, 42 kDa
Observed Molecular Weight 35-40 kDa, 80 kDa
GenBank Accession NumberBC047779
Gene Symbol TMEM173
Gene ID (NCBI) 340061
RRIDAB_10665370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

实验方案

Product Specific Protocols
WB protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IHC protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IF protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IP protocol for TMEM173/STING antibody 19851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.

Authors - Ting-Ting Chu
  • KO Validated
View Article
mouseWB

Nature

eccDNAs are apoptotic products with high innate immunostimulatory activity

Authors - Yuangao Wang
  • KO Validated
View Article
humanWB

Science

The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.

Authors - Mena Abdel-Nour
View Article
humanWB,IF

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
  • KD Validated
View Article
humanWB

Cancer Cell

Mutant p53 suppresses innate immune signaling to promote tumorigenesis.

Authors - Monisankar Ghosh
  • KD Validated
View Article
mouseIF

Nat Immunol

Homeostatic regulation of STING protein at the resting state by stabilizer TOLLIP.

Authors - Vladislav Pokatayev
View Article