CoraLite® Plus 488-conjugated TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-19851

Print datasheet

Synonyms

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173



经过测试的应用

Positive IF detected inHT-29 cells, HepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19851-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-19851 is TMEM173/STING Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF
Tested Reactivity human
Immunogen TMEM173/STING fusion protein Ag13921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 173
Synonyms FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173
Calculated Molecular Weight 379 aa, 42 kDa
GenBank Accession NumberBC047779
Gene Symbol TMEM173
Gene ID (NCBI) 340061
RRIDAB_2883086
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WV6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TMEM173/STING antibody CL488-19851Download protocol
FC protocol for CL Plus 488 TMEM173/STING antibody CL488-19851Download protocol
Standard Protocols
Click here to view our Standard Protocols