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验证数据展示

  • IF Staining of HT-29 using CL594-19851

    IF Staining of HT-29 using CL594-19851

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

  • FC experiment of THP-1 using CL594-19851

    FC experiment of THP-1 using CL594-19851

    1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL594-19851

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P, FC (Intra) and More (1)

别名

STING, NET23, MPYS, MITA, ERIS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inHT-29 cells, human tonsillitis tissue, HepG2 cells
Positive FC (Intra) detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19851-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-19851 targets TMEM173/STING in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 TMEM173/STING fusion protein Ag13921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 173
别名 STING, NET23, MPYS, MITA, ERIS
计算分子量 379 aa, 42 kDa
观测分子量 35-40 kDa, 80 kDa
GenBank蛋白编号BC047779
基因名称 STING
Gene ID (NCBI) 340061
RRIDAB_2919854
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WV6
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

实验方案

Product Specific Protocols
IF protocol for CL594 TMEM173/STING antibody CL594-19851Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Neuroinflammation

The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage

Authors - Xingfei Zhu
View Article
mouseFC

Mol Pharm

STING-Targeted PET Imaging for Specific Detection and Therapeutic Monitoring of Myocarditis

Authors - Zhou Ye
View Article