TMEM173/STING Recombinant antibody
TMEM173/STING Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
7K7
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MOLT-4 cells, Jurkat cells, K-562 cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human tonsillitis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | THP-1 cells, human tonsillitis tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80165-1-RR targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | TMEM173/STING fusion protein Ag13921 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | transmembrane protein 173 |
Synonyms | STING, NET23, MPYS, MITA, ERIS |
Calculated Molecular Weight | 379 aa, 42 kDa |
Observed Molecular Weight | 37 kDa |
GenBank Accession Number | BC047779 |
Gene Symbol | STING |
Gene ID (NCBI) | 340061 |
RRID | AB_2918870 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q86WV6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TMEM173/STING antibody 80165-1-RR | Download protocol |
IHC protocol for TMEM173/STING antibody 80165-1-RR | Download protocol |
IF protocol for TMEM173/STING antibody 80165-1-RR | Download protocol |
IP protocol for TMEM173/STING antibody 80165-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int Immunopharmacol Roflumilast attenuates neuroinflammation post retinal ischemia/reperfusion injury by regulating microglia phenotype via the Nrf2/STING/NF-κB pathway | ||
J Ethnopharmacol Lycopodium japonicum Thunb. inhibits chondrocyte apoptosis, senescence and inflammation in osteoarthritis through STING/NF-κB signaling pathway |