TMEM173/STING Recombinant antibody

TMEM173/STING Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

7K7

Cat no : 80165-1-RR

Print datasheet

Synonyms

STING, NET23, MPYS, MITA, ERIS



经过测试的应用

Positive WB detected inHeLa cells, MOLT-4 cells, Jurkat cells, K-562 cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inTHP-1 cells, human tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80165-1-RR targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen TMEM173/STING fusion protein Ag13921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transmembrane protein 173
Synonyms STING, NET23, MPYS, MITA, ERIS
Calculated Molecular Weight 379 aa, 42 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC047779
Gene Symbol STING
Gene ID (NCBI) 340061
RRIDAB_2918870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

实验方案

Product Specific Protocols
WB protocol for TMEM173/STING antibody 80165-1-RRDownload protocol
IHC protocol for TMEM173/STING antibody 80165-1-RRDownload protocol
IF protocol for TMEM173/STING antibody 80165-1-RRDownload protocol
IP protocol for TMEM173/STING antibody 80165-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

Roflumilast attenuates neuroinflammation post retinal ischemia/reperfusion injury by regulating microglia phenotype via the Nrf2/STING/NF-κB pathway

Authors - Yuyan Guo
human,mouseWB,IHC,IF

J Ethnopharmacol

Lycopodium japonicum Thunb. inhibits chondrocyte apoptosis, senescence and inflammation in osteoarthritis through STING/NF-κB signaling pathway

Authors - Jiawei Ma