验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80165-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL647-80165 targets TMEM173/STING in IF-P applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF-P Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | rat |
| 免疫原 | TMEM173/STING fusion protein Ag13921 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 173 |
| 别名 | FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, STING, TMEM173, transmembrane protein 173 |
| 计算分子量 | 379 aa, 42 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC047779 |
| 基因名称 | STING |
| Gene ID (NCBI) | 340061 |
| RRID | AB_2920332 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q86WV6 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 TMEM173/STING antibody CL647-80165 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |