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验证数据展示

  • FC experiment of Transfected HEK-293T using 98263-1-RR

    FC experiment of Transfected HEK-293T using 98263-1-RR

    1x10^6 HEK-293T cells or TMIGD2/CD28H transfected HEK-293T cells were stained with 0.25 ug Anti-Human TMIGD2/CD28H Rabbit Recombinant Antibody (98263-1-RR, Clone:241807A5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using 98263-1-RR

    FC experiment of Transfected HEK-293T using 98263-1-RR

    1x10^6 HEK-293T cells or TMIGD2/CD28H transfected HEK-293T cells were stained with 0.25 ug Anti-Human TMIGD2/CD28H Rabbit Recombinant Antibody (98263-1-RR, Clone: 241807A5) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98263-1-RR

    Affinity and Kinetic Characterization of 98263-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98263-1-RR against Human TMIGD2/CD28H were performed. The affinity constant is 171 nM.

Anti-Human TMIGD2/CD28H Rabbit Recombinant Antibody

TMIGD2/CD28H Uni-rAbTM Recombinant Antibody for FC
Cat No. 98263-1-RR

产品说明书

CloneNo. 241807A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98263-1-RR targets TMIGD2/CD28H in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane and immunoglobulin domain containing 2
别名 TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H
计算分子量 282 aa, 31 kDa
GenBank蛋白编号BC015655
基因名称 TMIGD2
Gene ID (NCBI) 126259
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96BF3
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704).

实验方案

Product Specific Protocols
FC protocol for TMIGD2/CD28H antibody 98263-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols