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PE Anti-Human TNFR1/CD120a Rabbit Recombinant Antibody

TNFR1/CD120a Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241337H6

Cat no : PE-98085

Print datasheet

Synonyms

CD120a, TNFR1, TNFRSF1A, 241337H6, p60

验证数据展示

  • FC experiment of human peripheral blood leukocytes using PE-98085

    FC experiment of human peripheral blood leukocytes using PE-98085

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human TNFR1/CD120a Rabbit Recombinant Antibody (PE-98085, Clone: 241337H6) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using PE-98085

    FC experiment of human peripheral blood leukocytes using PE-98085

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human TNFR1/CD120a Rabbit Recombinant Antibody (PE-98085, Clone: 241337H6) (red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98085 targets TNFR1/CD120a in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 1A
Synonyms CD120a, TNFR1, TNFRSF1A, 241337H6, p60
Calculated Molecular Weight 455 aa, 50 kDa
GenBank Accession NumberBC010140
Gene Symbol TNFR1
Gene ID (NCBI) 7132
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19438
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

实验方案

Product Specific Protocols
FC protocol for PE TNFR1/CD120a antibody PE-98085Download protocol
Standard Protocols
Click here to view our Standard Protocols