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TNFRSF9/CD137 Recombinant antibody, PBS Only (Detector)

TNFRSF9/CD137 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241006G5

Cat no : 83893-4-PBS

Print datasheet

Synonyms

TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor

验证数据展示

  • Sandwich ELISA standard curve of MP00870-2

    Sandwich ELISA standard curve of MP00870-2

    Sandwich ELISA standard curve of MP00870-2, Human TNFRSF9/CD137 Recombinant Matched Antibody Pair - PBS only. 83893-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0016. 83893-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Sample test of MP00870-2

    Sample test of MP00870-2

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean TNFRSF9 concentration was undetermined in unstimulated PBMC supernatant, 173.5 pg/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean TNFRSF9/CD137 concentration was undetermined in unstimulated PBMC supernatant, 435.7 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00870-2

    Sample test of MP00870-2

    Serum of fifteen individual healthy human donors was measured. The TNFRSF9/CD137 concentration of detected samples was determined to be 360.9 pg/mL with a range of 47.6-1,328.1 pg/mL.

  • Cytometric bead array standard curve of MP00870-2

    Cytometric bead array standard curve of MP00870-2

    Cytometric bead array standard curve of MP00870-2, TNFRSF9/CD137 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83893-1-PBS. Detection antibody: 83893-4-PBS. Standard: Eg0016. Range: 0.313-40 ng/mL.

产品信息

83893-4-PBS targets TNFRSF9/CD137 as part of a matched antibody pair:

MP00870-2: 83893-1-PBS capture and 83893-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 9
Synonyms TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC006196
Gene Symbol TNFRSF9/CD137
Gene ID (NCBI) 3604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07011
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)