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验证数据展示

  • FC experiment of Jurkat using CL488-68170

    FC experiment of Jurkat using CL488-68170

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TOLLIP (CL488-68170, Clone:1B3C4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TOLLIP Monoclonal antibody

TOLLIP Monoclonal Antibody for FC (Intra)
Cat No. CL488-68170

Print datasheet

CloneNo. 1B3C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 1B3C4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy TOLLIP Ready-To-Use IHC Kit. TOLLIP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68170-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68170 targets TOLLIP in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen TOLLIP fusion protein Ag32731 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name toll interacting protein
Synonyms Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 1B3C4
Calculated Molecular Weight 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC012057
Gene Symbol TOLLIP
Gene ID (NCBI) 54472
RRIDAB_3084429
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H0E2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TOLLIP antibody CL488-68170Download protocol
Standard Protocols
Click here to view our Standard Protocols