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验证数据展示

  • FC experiment of Jurkat using CL488-68170

    FC experiment of Jurkat using CL488-68170

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TOLLIP (CL488-68170, Clone:1B3C4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TOLLIP Monoclonal antibody

TOLLIP Monoclonal Antibody for FC (Intra)
Cat No. CL488-68170

产品说明书

CloneNo. 1B3C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 1B3C4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy TOLLIP Ready-To-Use IHC Kit. TOLLIP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68170-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68170 targets TOLLIP in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 TOLLIP fusion protein Ag32731 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 toll interacting protein
别名 Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 1B3C4
计算分子量 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC012057
基因名称 TOLLIP
Gene ID (NCBI) 54472
RRIDAB_3084429
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H0E2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TOLLIP antibody CL488-68170Download protocol
Standard Protocols
Click here to view our Standard Protocols