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CoraLite®488-conjugated TOM20 Polyclonal antibody

TOM20 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG


human, mouse, rat


FC (Intra), IF


CoraLite®488 Fluorescent Dye

Cat No : CL488-11802

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KIAA0016, MAS20, Mitochondrion Marker, MOM19, TOM20, TOMM20


Positive IF detected inHUVEC cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11802-1-AP


Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery


IFSee 1 publications below


CL488-11802 targets TOM20 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOM20 fusion protein Ag2378 种属同源性预测
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Synonyms KIAA0016, MAS20, Mitochondrion Marker, MOM19, TOM20, TOMM20
Calculated molecular weight 145 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC000882
Gene symbol TOMM20
Gene ID (NCBI) 9804
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.



 TOM20 (KIAA0016) belongs to the Tom family. It is a subunit of the mitochondrial import receptor (PMID: 7584026), whose main role is to translocate cytosolically synthesized mitochondrial proteins through the outer mitochondrial membrane and subsequently facilitate the movement of proteins into the TOM40 translocation pore complex (PMID: 21173275).

 What is the molecular weight of TOM20? 

 It is a short 16.3 kDa protein containing several highly conserved regions, including the transmembrane segment, the ligand-binding domain, and flexible segments at the N terminus and the C terminus of the protein crucial for its function (PMID: 15733919).

 What is the subcellular localization of TOM20?

 It specifically localizes in the mitochondrial outer membrane. In malignant cells, strong granular staining in the cytoplasm has been observed.

 What is the tissue specificity of TOM20?

 It is ubiquitously expressed in various tissues, with a particularly high expression in the brain, thyroid, and pancreas.

 What is the function of TOM20 in the mitochondrial membrane?

 Mitochondrial preproteins are generally synthesized in the cytoplasm with signal or targeting sequences, which are recognized by specific receptors on the outer mitochondrial membrane. TOM20, as one of the components of the TOM40 complex, specifically recognizes pre-sequences on target proteins or their unfolded forms. In addition to its translocase activity, TOM20 may act as a chaperone preventing these proteins from aggregation at the surface of mitochondria (PMID: 14699115).

 What is TOM20's involvement in disease?

 Diseases linked to the misregulation of TOM20 include Perry Syndrome and neurodegeneration with brain iron accumulation 2A. Numerous studies also associate deregulation of TOM20 with an array of mitochondrial dysfunctions and mitophagy defects (PMIDs: 30254015, 30160596). 


Product Specific Protocols
IF protocol for CL488 TOM20 antibody CL488-11802Download protocol
FC protocol for CL488 TOM20 antibody CL488-11802Download protocol
Standard Protocols
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Free Radic Biol Med

Loss of the redox mitochondrial protein mitoNEET leads to mitochondrial dysfunction in B-cell acute lymphoblastic leukemia.

Authors - Werner J Geldenhuys