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验证数据展示

  • WB analysis using CL594-66562

    WB analysis using CL594-66562

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66562 (TOMM22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using CL594-66562

    FC experiment of HEK-293 using CL594-66562

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Tom22 (CL594-66562, Clone:1C2B11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Tom22 Monoclonal antibody

Tom22 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL594-66562

产品说明书

CloneNo. 1C2B11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra)

别名

TOMM22, MSTP065, MST065, hTom22, 1C9 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66562-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66562 targets Tom22 in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原 Tom22 fusion protein Ag1797 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 22 homolog (yeast)
别名 TOMM22, MSTP065, MST065, hTom22, 1C9 2
计算分子量 15 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009363
基因名称 TOMM22/Tom22
Gene ID (NCBI) 56993
RRIDAB_2920005
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS69
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOMM22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

实验方案

Product Specific Protocols
WB protocol for CL594 Tom22 antibody CL594-66562Download protocol
FC protocol for CL594 Tom22 antibody CL594-66562Download protocol
Standard Protocols
Click here to view our Standard Protocols