CoraLite®555-conjugated Tom22 Monoclonal antibody
Tom22 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
1C2B11
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66562-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-66562 targets Tom22 in IF applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | Tom22 fusion protein Ag1797 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | translocase of outer mitochondrial membrane 22 homolog (yeast) |
Synonyms | 1C9 2, hTom22, MST065, MSTP065, TOM22, TOMM22 |
Calculated Molecular Weight | 15 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC009363 |
Gene Symbol | TOMM22/Tom22 |
Gene ID (NCBI) | 56993 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9NS69 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises the 22 kDa protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).
实验方案
Product Specific Protocols | |
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IF protocol for CL555 Tom22 antibody CL555-66562 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |