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TP63 Monoclonal antibody

TP63 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4D6G12

Cat no : 60332-2-Ig

Print datasheet

Synonyms

p63, CUSP, tumor protein p63, Tumor protein 63, Transformation-related protein 63

验证数据展示

  • WB analysis of HaCaT using 60332-2-Ig

    WB analysis of HaCaT using 60332-2-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skin using 60332-2-Ig

    IHC staining of rat skin using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IHC staining of human tonsillitis using 60332-2-Ig

    IHC staining of human tonsillitis using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • WB analysis of A431 using 60332-2-Ig

    WB analysis of A431 using 60332-2-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 60332-2-Ig

    IHC staining of mouse skin using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • WB analysis of U-937 using 60332-2-Ig

    WB analysis of U-937 using 60332-2-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60332-2-Ig

    IHC staining of human tonsillitis using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IHC staining of human prostate cancer using 60332-2-Ig

    IHC staining of human prostate cancer using 60332-2-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included

经过测试的应用

Positive WB detected inHaCaT cells, A431 cells, U-937 cells
Positive IHC detected inrat skin tissue, human prostate cancer tissue, human tonsillitis tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TP63 Ready-To-Use IHC Kit. TP63 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60332-2-Ig targets TP63 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TP63 fusion protein Ag2791 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name tumor protein p63
Synonyms p63, CUSP, tumor protein p63, Tumor protein 63, Transformation-related protein 63
Calculated Molecular Weight 680 aa, 77 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC039815
Gene Symbol TP63
Gene ID (NCBI) 8626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H3D4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

实验方案

Product Specific Protocols
WB protocol for TP63 antibody 60332-2-IgDownload protocol
IHC protocol for TP63 antibody 60332-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dose Response

Scutellarin Inhibits Glioblastoma Growth in a Dose-dependent Manner by Suppressing the p63 Signaling Pathway

Authors - Yongjie Chen
View Article