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  • Featured Product
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CoraLite® Plus 488-conjugated TPPII Polyclonal antibody

TPPII Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-14120

Print datasheet

Synonyms

TPP 2, TPP II, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, Tripeptidyl peptidase II

验证数据展示

  • IF Staining of HepG2 using CL488-14120

    IF Staining of HepG2 using CL488-14120

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 TPPII antibody (CL488-14120) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 14120-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14120 targets TPPII in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen TPPII fusion protein Ag5296 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripeptidyl peptidase II
Synonyms TPP 2, TPP II, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, Tripeptidyl peptidase II
Calculated Molecular Weight 1249 aa, 138 kDa
Observed Molecular Weight 135-140 kDa
GenBank Accession NumberBC039905
Gene Symbol TPP2
Gene ID (NCBI) 7174
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29144
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TPPII antibody CL488-14120Download protocol
FC protocol for CL Plus 488 TPPII antibody CL488-14120Download protocol
Standard Protocols
Click here to view our Standard Protocols