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  • Featured Product
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TPT1 Monoclonal antibody

TPT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G6A10

Cat no : 66713-1-Ig

Print datasheet

Synonyms

TCTP, p23, HRF, Histamine-releasing factor, Fortilin

验证数据展示

  • WB analysis using 66713-1-Ig

    WB analysis using 66713-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66713-1-Ig

    WB analysis of MCF-7 using 66713-1-Ig

    WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells.

  • IHC staining of human colon cancer using 66713-1-Ig

    IHC staining of human colon cancer using 66713-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPT1 antibody included
  • IF Staining of HEK-293 using 66713-1-Ig

    IF Staining of HEK-293 using 66713-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HEK-293 using 66713-1-Ig

    IF Staining of HEK-293 using 66713-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, RAW 264.7 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TPT1 Ready-To-Use IHC Kit. TPT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66713-1-Ig targets TPT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TPT1 fusion protein Ag27651 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor protein, translationally-controlled 1
Synonyms TCTP, p23, HRF, Histamine-releasing factor, Fortilin
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003352
Gene Symbol TPT1
Gene ID (NCBI) 7178
RRIDAB_2882064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13693
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.

实验方案

Product Specific Protocols
WB protocol for TPT1 antibody 66713-1-IgDownload protocol
IHC protocol for TPT1 antibody 66713-1-IgDownload protocol
IF protocol for TPT1 antibody 66713-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols