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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated TPT1 Monoclonal antibody

TPT1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1G6A10

Cat no : CL555-66713

Print datasheet

Synonyms

FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1

验证数据展示

  • IF Staining of HEK-293 using CL555-66713

    IF Staining of HEK-293 using CL555-66713

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CoraLite®555 TPT1 antibody (CL555-66713, Clone: 1G6A10 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TPT1 Ready-To-Use IHC Kit. TPT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66713-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66713 targets TPT1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen TPT1 fusion protein Ag27651 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor protein, translationally-controlled 1
Synonyms FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003352
Gene Symbol TPT1
Gene ID (NCBI) 7178
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13693
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.

实验方案

Product Specific Protocols
IF protocol for CL555 TPT1 antibody CL555-66713Download protocol
Standard Protocols
Click here to view our Standard Protocols