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  • Featured Product
  • KD/KO Validated

TPT1 Monoclonal antibody, PBS Only

TPT1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1G6A10

Cat No : 66713-1-PBS

Print datasheet

Synonyms

FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1

验证数据展示

  • WB analysis using 66713-1-Ig (same clone as 66713-1-PBS)

    WB analysis using 66713-1-Ig (same clone as 66713-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66713-1-Ig (same clone as 66713-1-PBS)

    WB analysis of MCF-7 using 66713-1-Ig (same clone as 66713-1-PBS)

    WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66713-1-Ig (same clone as 66713-1-PBS)

    IHC staining of human colon cancer using 66713-1-Ig (same clone as 66713-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPT1 antibody included
  • IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

    IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

    IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TPT1 Ready-To-Use IHC Kit. TPT1 primary antibody included.

产品信息

66713-1-PBS targets TPT1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen TPT1 fusion protein Ag27651 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor protein, translationally-controlled 1
Synonyms FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003352
Gene Symbol TPT1
Gene ID (NCBI) 7178
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13693
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.