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验证数据展示

  • IF Staining of HepG2 using CL488-10325

    IF Staining of HepG2 using CL488-10325

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TRAP1 antibody (CL488-10325) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-10325

    FC experiment of HeLa using CL488-10325

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TRAP1 (CL488-10325) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TRAP1 Polyclonal antibody

TRAP1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10325

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10325-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10325 targets TRAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 TRAP1 fusion protein Ag0400 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TNF receptor-associated protein 1
别名 TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75
计算分子量 80 kDa
观测分子量 66-70 kDa, 60 kDa
GenBank蛋白编号BC000406
基因名称 TRAP1
Gene ID (NCBI) 10131
RRIDAB_2918971
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12931
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRAP1 antibody CL488-10325Download protocol
FC protocol for CL Plus 488 TRAP1 antibody CL488-10325Download protocol
Standard Protocols
Click here to view our Standard Protocols