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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TRIM14 Polyclonal antibody

TRIM14 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15742

Print datasheet

Synonyms

Tripartite motif-containing protein 14, tripartite motif containing 14, TRIM, KIAA0129

验证数据展示

  • IF Staining of HeLa using CL488-15742

    IF Staining of HeLa using CL488-15742

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 TRIM14 antibody (CL488-15742) at dilution of 1:200.

  • FC experiment of HeLa using CL488-15742

    FC experiment of HeLa using CL488-15742

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Trim14 Polyclonal Antibody (CL488-15742)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 15742-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15742 targets TRIM14 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen TRIM14 fusion protein Ag8158 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 14
Synonyms Tripartite motif-containing protein 14, tripartite motif containing 14, TRIM, KIAA0129
Calculated Molecular Weight 442 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC006333
Gene Symbol TRIM14
Gene ID (NCBI) 9830
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14142
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRIM14 antibody CL488-15742Download protocol
FC protocol for CL Plus 488 TRIM14 antibody CL488-15742Download protocol
Standard Protocols
Click here to view our Standard Protocols